Consensus sequence design as a general strategy to create hyperstable, biologically active proteins.

ABSTRACT: Consensus sequence design offers a promising strategy for designing proteins of high stability while retaining biological activity since it draws upon an evolutionary history in which residues important for both stability and function are likely to be conserved. Although there have been several reports of successful consensus design of individual targets, it is unclear from these anecdotal studies how often this approach succeeds and how often it fails. Here, we attempt to assess generality by designing consensus sequences for a set of six protein families with a range of chain lengths, structures, and activities. We characterize the resulting consensus proteins for stability, structure, and biological activities in an unbiased way. We find that all six consensus proteins adopt cooperative

SUBMITTER: Sternke M 

PROVIDER: S-EPMC6561275 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature