Evaluation of chloroplast genome annotation tools and application to analysis of the evolution of coffee species.

ABSTRACT: Chloroplast sequences are widely used for phylogenetic analysis due to their high degree of conservation in plants. Whole chloroplast genomes can now be readily obtained for plant species using new sequencing methods, giving invaluable data for plant evolution However new annotation methods are required for the efficient analysis of this data to deliver high quality phylogenetic analyses. In this study, the two main tools for chloroplast genome annotation were compared. More consistent detection and annotation of genes were produced with GeSeq when compared to the currently used Dogma. This suggests that the annotation of most of the previously annotated chloroplast genomes should now be updated. GeSeq was applied to species related to coffee, including 16 species of the Coffea and Psilant

SUBMITTER: Guyeux C 

PROVIDER: S-EPMC6561552 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature