DamC reveals principles of chromatin folding in vivo without crosslinking and ligation.

ABSTRACT: Current understanding of chromosome folding is largely reliant on chromosome conformation capture (3C)-based experiments, where chromosomal interactions are detected as ligation products after chromatin crosslinking. To measure chromosome structure in vivo, quantitatively and without crosslinking and ligation, we implemented a modified version of DNA adenine methyltransferase identification (DamID) named DamC, which combines DNA methylation-based detection of chromosomal interactions with next-generation sequencing and biophysical modeling of methylation kinetics. DamC performed in mouse embryonic stem cells provides the first in vivo validation of the existence of topologically associating domains (TADs), CTCF loops and confirms 3C-based measurements of the scaling of contact probabilitie

SUBMITTER: Redolfi J 

PROVIDER: S-EPMC6561777 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature