Role of the global regulator Rex in control of NAD⁺ -regeneration in Clostridioides (Clostridium) difficile.

ABSTRACT: For the human pathogen Clostridioides (also known as Clostridium) difficile, the ability to adapt to nutrient availability is critical for its proliferation and production of toxins during infection. Synthesis of the toxins is regulated by the availability of certain carbon sources, fermentation products and amino acids (e.g. proline, cysteine, isoleucine, leucine and valine). The effect of proline is attributable at least in part to its role as an inducer and substrate of D-proline reductase (PR), a Stickland reaction that regenerates NAD⁺ from NADH. Many Clostridium spp. use Stickland metabolism (co-fermentation of pairs of amino acids) to generate ATP and NAD⁺ . Synthesis of PR is activated by PrdR, a proline-responsive regulatory protein. Here we report that PrdR,

SUBMITTER: Bouillaut L 

PROVIDER: S-EPMC6561804 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature