In vivo imaging of type 1 diabetes immunopathology using eye-transplanted islets in NOD mice.

ABSTRACT:

Aims/hypothesis

Autoimmune attack against the insulin-producing beta cells in the pancreatic islets results in type 1 diabetes. However, despite considerable research, details of the type 1 diabetes immunopathology in situ are not fully understood mainly because of difficult access to the pancreatic islets in vivo.

Methods

Here, we used direct non-invasive confocal imaging of islets transplanted in the anterior chamber of the eye (ACE) to investigate the anti-islet autoimmunity in NOD mice before, during and after diabetes onset. ACE-transplanted islets allowed longitudinal studies of the autoimmune attack against islets and revealed the infiltration kinetics and in situ motility dynamics of fluorescence-labelled autoreactive T cells during diabetes development. Ex vivo immu

SUBMITTER: Abdulreda MH 

PROVIDER: S-EPMC6561836 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature