IgE re-programs alternatively-activated human macrophages towards pro-inflammatory anti-tumoural states.

ABSTRACT:

Background

Antibody Fc-driven engagement of macrophages is critical for evoking cellular activation and effector functions and influencing tumour-associated macrophage (TAM) recruitment. We previously reported that IgE class antibodies promote restriction of cancer growth in rodent models associated with significant TAM infiltration. However, the human macrophage-associated IgE-Fc Receptor (FcεR) axis remains unexplored. We investigated the effects of anti-tumour IgE stimulation on human macrophage activation.

Methods

Human blood monocyte-differentiated quiescent (M0), classically-(M1) and alternatively-(M2) activated macrophages were crosslinked with IgE and polyclonal antibodies to mimic immune complex formation. We examined surface marker expression, cytokine secretion, p

SUBMITTER: Pellizzari G 

PROVIDER: S-EPMC6562024 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature