APOA1 oxidation is associated to dysfunctional high-density lipoproteins in human abdominal aortic aneurysm.

ABSTRACT:

Background

High-density lipoproteins (HDL) are a complex mixture of lipids and proteins with vasculoprotective properties. However, HDL components could suffer post-translational modifications (PTMs) under pathological conditions, leading to dysfunctional HDL. We studied whether HDL are modified in abdominal aortic aneurysm (AAA) and the effect on HDL functionality.

Methods

HDL were isolated by ultracentrifugation from AAA tissue (HDL-T) and from plasma of healthy volunteers and then incubated with AAA tissue-conditioned medium (HDL-AAA CM). PTMs from these particles were characterized using Comet-PTM. The ability of HDL-AAA CM for promoting cholesterol efflux was determined ex vivo and in vivo by using J774A.1 [³H]cholesterol-labeled mouse macrophages and after i

SUBMITTER: Martinez-Lopez D 

PROVIDER: S-EPMC6562066 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature