Seno-destructive smooth muscle cells in the ascending aorta of patients with bicuspid aortic valve disease.

ABSTRACT:

Background

Ascending aortic aneurysms constitute an important hazard for individuals with a bicuspid aortic valve (BAV). However, the processes that degrade the aortic wall in BAV disease remain poorly understood.

Methods

We undertook in situ analysis of ascending aortas from 68 patients, seeking potentially damaging cellular senescence cascades. Aortas were assessed for senescence-associated-ß-galactosidase activity, p16^Ink4a and p21 expression, and double-strand DNA breaks. The senescence-associated secretory phenotype (SASP) of cultured-aged BAV aortic smooth muscle cells (SMCs) was evaluated by transcript profiling and consequences probed by combined immunofluorescence and circular polarization microscopy. The contribution of p38 MAPK signaling was assessed by

SUBMITTER: Balint B 

PROVIDER: S-EPMC6562112 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature