Establishment of a highly efficient conjugation protocol for Streptomyces kanamyceticus ATCC12853.

ABSTRACT: Kanamycin B as the secondary metabolite of wild-type Streptomyces kanamyceticus (S. kanamyceticus) ATCC12853 is often used for the synthesis of dibekacin and arbekacin. To construct the strain has the ability for kanamycin B production; the pSET152 derivatives from Escherichia coli ET12567 were introduced to S. kanamyceticus by intergeneric conjugal transfer. In this study, we established a reliable genetic manipulation system for S. kanamyceticus. The key factors of conjugal transfer were evaluated, including donor-to-recipient ratio, heat-shock, and the overlaying time of antibiotics. When spores were used as recipient, the optimal conjugation frequency was up to 6.7 × 10^-6 . And mycelia were used as an alternative recipient for conjugation instead of spores; the most suitable

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6562128 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature