Endothelial FAM3A positively regulates post-ischaemic angiogenesis.

ABSTRACT:

Background

Angiogenesis improves reperfusion to the ischaemic tissue after vascular obstruction. The underlying molecular mechanisms of post-ischaemic angiogenesis are not clear. FAM3A belongs to the family with sequence similarity 3 (FAM3) genes, but its biological function in endothelial cells in regards to vascular diseases is not well understood.

Methods

Gain- and loss-of-function methods by adenovirus or associated-adenovirus (AAV) in different models were applied to investigate the effects of FAM3A on endothelial angiogenesis. Endothelial angiogenesis was analysed by tube formation, migration and proliferation in vitro, and the blood flow and capillary density in a hind limb ischaemic model in vivo.

Findings

Endothelial FAM3A expression is downregulated under hy

SUBMITTER: Xu W 

PROVIDER: S-EPMC6562148 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature