Roles of end-binding 1 protein and gamma-tubulin small complex in cytokinesis and flagella formation of Giardia lamblia.

ABSTRACT: Giardia lamblia is a unicellular organism with two nuclei, a median body, eight flagella, and an adhesive disk. γ-Tubulin is a microtubule (MT)-nucleating protein that functions in the γ-tubulin small complex (γ-TuSC) in budding yeast. In this study, G. lamblia γ-tubulin (Glγ-tubulin) was found to bind to another MT-binding protein, namely G. lamblia end-binding protein 1 (GlEB1), via both in vivo and in vitro assays. Hemagglutinin (HA)-tagged Glγ-tubulin localized to the basal bodies, axonemes, and median bodies of G. lamblia trophozoites. The knockdown of Glγ-tubulin expression using an anti-Glγ-tubulin morpholino resulted in a decreased growth rate and an increased failed cytokinesis cells of Giardia. The formation of median bodies was affected, and the central pair of MTs in flagella w

SUBMITTER: Kim J 

PROVIDER: S-EPMC6562232 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature