Membrane Protein Identification in Rodent Brain Tissue Samples and Acute Brain Slices.

ABSTRACT: Acute brain slices are a sample format for electrophysiology, disease modeling, and organotypic cultures. Proteome analyses based on mass spectrometric measurements are seldom used on acute slices, although they offer high-content protein analyses and explorative approaches. In neuroscience, membrane proteins are of special interest for proteome-based analysis as they are necessary for metabolic, electrical, and signaling functions, including myelin maintenance and regeneration. A previously published protocol for the enrichment of plasma membrane proteins based on aqueous two-phase polymer systems followed by mass spectrometric protein identification was adjusted to the small sample size of single acute murine slices from newborn animals and the reproducibility of the results was analyzed

SUBMITTER: Joost S 

PROVIDER: S-EPMC6562397 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature