Observation of Dynamic Cellular Migration of the Medial Edge Epithelium of the Palatal Shelf in vitro.

ABSTRACT: Palatal fusion is a critical step during palatogenesis. In this fusing interface, the epithelial sheets need to be removed in order to achieve mesenchymal continuity. Epithelial cellular migration is one of the possible mechanisms, and live imaging of the labeled epithelium could provide direct evidence for it. However, the removal of medial edge epithelium (MEE) between the bilateral processes takes place in the middle of the dorso-ventral axis of the palatal shelf, and thus it is challenging to capture the cellular behavior directly. Here, we evaluate cellular behavior of MEE cells using a live imaging technique with a mouse model which expresses GFP under the promoter of Keratin14 (K14-GFP) and unpaired palatal shelf culture. Using this approach, we successfully obtained live images of

SUBMITTER: Aoyama G 

PROVIDER: S-EPMC6562562 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature