Full-Length Multi-Barcoding: DNA Barcoding from Single Ingredient to Complex Mixtures.

ABSTRACT: DNA barcoding has been used for decades, although it has mostly been applied to somesingle-species. Traditional Chinese medicine (TCM), which is mainly used in the form ofcombination-one type of the multi-species, identification is crucial for clinical usage.Next-generation Sequencing (NGS) has been used to address this authentication issue for the pastfew years, but conventional NGS technology is hampered in application due to its short sequencingreads and systematic errors. Here, a novel method, Full-length multi-barcoding (FLMB) vialong-read sequencing, is employed for the identification of biological compositions in herbalcompound formulas in adequate and well controlled studies. By directly sequencing the full-lengthamplicons of ITS2 and psbA-trnH through single-molecule real-time (SM

SUBMITTER: Zhang P 

PROVIDER: S-EPMC6562688 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature