Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived Xenograft Mouse Model.

ABSTRACT: Cholangiocarcinoma (CCA) is a deadly malignant tumor of the liver. It is a significant health problem in Thailand. The critical obstacles of CCA diagnosis and treatment are the high heterogeneity of disease and considerable resistance to treatment. Recent multi-omics studies revealed the promising targets for CCA treatment; however, limited models for drug discovery are available. This study aimed to develop a patient-derived xenograft (PDX) model as well as PDX-derived cell lines of CCA for future drug screening. From a total of 16 CCA frozen tissues, 75% (eight intrahepatic and four extrahepatic subtypes) were successfully grown and subpassaged in Balb/c Rag-2^-/-/Jak3^-/- mice. A shorter duration of PDX growth was observed during F0 to F2 transplantation; concomitant

SUBMITTER: Vaeteewoottacharn K 

PROVIDER: S-EPMC6562875 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature