CD8⁺ PD-1⁺ T-cells and PD-L1⁺ circulating tumor cells in chemotherapy-naive non-small cell lung cancer: towards their clinical relevance?

ABSTRACT:

Background

Since tumor cells may escape from immune surveillance through the programmed cell death 1 (PD-1)/programmed death ligand (PD-L)1 axis, this study was designed in order to evaluate whether there is a correlation between the levels of PD-1⁺ and PD-L1⁺-expressing immune cells (ICs) and circulating tumor cells (CTCs) in patients with non-small cell lung cancer (NSCLC).

Patients and methods

Peripheral blood was obtained from 37 chemotherapy-naïve patients with metastatic NSCLC before treatment. PD-1 and PD-L1 expression was evaluated (1) on ICs with anti-tumor function (CD4⁺ and CD8⁺ T-cells, B-cells, monocytes/dendritic cells) using flow cytometry, (2) on CTCs by immunofluorescence and (3) on cells from tumor tissues by immu

SUBMITTER: Kotsakis A 

PROVIDER: S-EPMC6563396 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature