CTX-M type extended-spectrum β-lactamase in Escherichia coli isolated from extra-intestinal infections in a tertiary care hospital in south India.

ABSTRACT:

Background & objectives

Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli carrying bla_CTX-M genes have been spreading globally, but there are geographical variations in the type of bla_CTX-Mgenes prevalent and there are scanty data from India. This study was conducted to determine the CTX-M type ESBLs in E. coli isolates obtained from clinical specimens from patients with extra-intestinal infections attending a tertiary care hospital in south India.

Methods

ESBL-producing E. coli isolated from patients with extra-intestinal infections were subjected to PCR using CTX-M group-specific primers. From a representative isolate, full-length CTX-M-15 gene was amplified and sequenced. An internal fragment of this gene was sequenced in 10 representative isolates.

Results

Of the 300 isolates of E. coli tested, 88 per cent carried CTX-M genes and bla_CTX-M-15was the most dominant gene present in 90 per cent of the positive isolates. Most (91%) of the isolates positive for bla_CTX-M were sensitive to meropenem.

Interpretation & conclusions

Our findings showed bla_CTX-M-15 to be the dominant gene. Based on the data on antimicrobial susceptibility, cefoperazone-sulbactum could be an antimicrobial of choice.

SUBMITTER: Rohit A 

PROVIDER: S-EPMC6563725 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature