Injection parameters and virus dependent choice of promoters to improve neuron targeting in the nonhuman primate brain.

ABSTRACT: We, like many others, wish to use modern molecular methods to alter neuronal functionality in primates. For us, this requires expression in a large proportion of the targeted cell population. Long generation times make germline modification of limited use. The size and intricate primate brain anatomy poses additional challenges. We surved methods using lentiviruses and serotypes of adeno-associated viruses (AAVs) to introduce active molecular material into cortical and subcortical regions of old-world monkey brains. Slow injections of AAV2 give well-defined expression of neurons in the cortex surrounding the injection site. Somewhat surprisingly we find that in the monkey the use of cytomegalovirus promoter in lentivirus primarily targets glial cells but few neurons. In contrast, with a sy

SUBMITTER: Lerchner W 

PROVIDER: S-EPMC6563815 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature