Cell type transcriptome atlas for the planarian Schmidtea mediterranea.

ABSTRACT: The transcriptome of a cell dictates its unique cell type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal: the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. We generated data for 66,783 cells, defining transcriptomes for known and many previously unknown planarian cell types and for putative transition states between stem and differentiated cells. We also uncovered regionally expressed genes in muscle, which harbors positional information. Identifying the transcriptomes for po

SUBMITTER: Fincher CT 

PROVIDER: S-EPMC6563842 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature