Microbiota data from low biomass milk samples is markedly affected by laboratory and reagent contamination.

ABSTRACT: Discoveries of bacterial communities in environments that previously have been described as sterile have in recent years radically challenged the view of these environments. In this study we aimed to use 16S rRNA sequencing to describe the composition and temporal stability of the bacterial microbiota in bovine milk from healthy udder quarters, an environment previously believed to be sterile. Sequencing of the 16S rRNA gene is a technique commonly used to describe bacterial composition and diversity in various environments. With the increased use of 16S rRNA gene sequencing, awareness of methodological pitfalls such as biases and contamination has increased although not in equal amount. Evaluation of the composition and temporal stability of the microbiota in 288 milk samples was largely

SUBMITTER: Dahlberg J 

PROVIDER: S-EPMC6564671 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature