Synaptotagmin 5 regulates Ca²⁺-dependent Weibel-Palade body exocytosis in human endothelial cells.

ABSTRACT: Elevations of intracellular free Ca²⁺ concentration ([Ca²⁺]_i) are a potent trigger for Weibel-Palade body (WPB) exocytosis and secretion of von Willebrand factor (VWF) from endothelial cells; however, the identity of WPB-associated Ca²⁺-sensors involved in transducing acute increases in [Ca²⁺]_i into granule exocytosis remains unknown. Here, we show that synaptotagmin 5 (SYT5) is expressed in human umbilical vein endothelial cells (HUVECs) and is recruited to WPBs to regulate Ca²⁺-driven WPB exocytosis. Western blot analysis of HUVECs identified SYT5 protein, and exogenously expressed SYT5-mEGFP localised almost exclusively to WPBs. shRNA-mediated knockdown of endogenous SYT5 (shSYT5) reduced the rate and extent of

SUBMITTER: Lenzi C 

PROVIDER: S-EPMC6565431 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature