PICS: a platform for planar imaging of curved surfaces of brain and other tissue.

ABSTRACT: Optical imaging of wholemount tissue samples provides greater understanding of structure-function relationships as the architecture of these specimens is generally well preserved. However, difficulties arise when attempting to stitch together images of multiple regions of larger, oddly shaped specimens. These difficulties include (1) maintaining consistent signal-to-noise ratios when the overlying sample surface is uneven, (2) ensuring sample viability when live samples are required, and (3) stabilizing the specimen in a fixed position in a flowing medium without distorting the tissue sample. To address these problems, we designed a simple and cost-efficient device that can be 3D-printed and machined. The design for the device, named the Platform for Planar Imaging of Curved Surfaces (PICS

SUBMITTER: Scoggin JL 

PROVIDER: S-EPMC6565470 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature