Next Generation Sequencing for Long Non-coding RNAs Profile for CD4⁺ T Cells in the Mouse Model of Acute Asthma.

ABSTRACT:

Background and aims

Although long non-coding RNAs (lncRNAs) have been linked to many diseases including asthma, little is known about lncRNA transcriptomes of CD4⁺ T cells in asthma. The present study aimed to explore the lncRNAs profile in the CD4⁺T cells from the mouse model of acute asthma.

Methods

Next generation sequencing for lncRNAs and mRNAs was performed on CD4+ T cells from asthma and control mice. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway analyses were performed to predict the functions and signal pathways for the aberrant lncRNAs. The selected lncRNAs were further measured using quantitative real-time PCR (polymerase chain reaction) and observed in the fluorescence in situ hybridization (FISH). The

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6565949 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature