PCYOs: Binary vectors for simple visible selection of transformants using an albino-cotyledon mutant in Arabidopsis thaliana.

ABSTRACT: Several selection markers for the screening of transformants have been developed; however, simple and reliable methods are generally preferred. We have developed a novel visible selection system for the identification of transformants in Arabidopsis thaliana that does not require any special reagent and/or equipment except using the albino-cotyledon mutant cyo1. In this system, the pCYO vector carrying the CYO1 genomic fragment as a selection marker is introduced into the cyo1 mutant. Transformation is performed by the Agrobacterium-mediated floral dip method and resultant T1 seeds are sown in soil. Seedlings with green cotyledons, not albino, are expected to be 'complemented' transformants with the transgene of interest. This system provides a very simple selection method that can be perf

SUBMITTER: Yamatani H 

PROVIDER: S-EPMC6566005 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature