Probing and regulating the activity of cellular enzymes by using DNA tetrahedron nanostructures.

ABSTRACT: Given the essential role of apurinic/apyrimidinic endonuclease (APE1) in gene repair and cancer progression, we report a novel approach for probing and regulating cellular APE1 activity by using DNA tetrahedrons. The tetrahedron with an AP site-containing antenna exhibits high sensitivity and specificity to APE1. It is suitable for APE1 in vitro detection (detection limit 5 pM) and cellular fluorescence imaging without any auxiliary transfection reagents, which discriminates the APE1 expression level of cancer cells and normal cells. In contrast, the tetrahedron with an AP site on its scaffold exhibits high binding affinity to APE1 but limits enzymatic catalysis making this nanostructure an APE1 inhibitor with an IC₅₀ of 14.8 nM. It suppresses the APE1 activity in living

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6566069 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature