Extra Purified Exosomes from Human Placenta Contain An Unpredictable Small Number of Different Major Proteins.

ABSTRACT: Exosomes are nanovesicles (30-100 nm) containing various RNAs and different proteins. Exosomes are important in intracellular communication, immune function, etc. Exosomes from different sources including placenta were mainly obtained by different types of centrifugation and ultracentrifugations and were reported to contain from a few dozen to thousands of different proteins. First crude exosome preparations from four placentas (normal pregnancy) were obtained here using several standard centrifugations but then were additionally purified by gel filtration on Sepharose 4B. Individual preparations demonstrated different gel filtration profiles showing good or bad separation of exosome peaks from two peaks of impurity proteins and their complexes. According to electron microscopy, exosomes b

SUBMITTER: Burkova EE 

PROVIDER: S-EPMC6566543 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature