Surface Orientation and Binding Strength Modulate Shape of FtsZ on Lipid Surfaces.

ABSTRACT: We have used a simple model system to test the prediction that surface attachment strength of filaments presenting a torsion would affect their shape and properties. FtsZ from E. coli containing one cysteine in position 2 was covalently attached to a lipid bilayer containing maleimide lipids either in their head group (to simulate tight attachment) or at the end of a polyethylene glycol molecule attached to the head group (to simulate loose binding). We found that filaments tightly attached grew straight, growing from both ends, until they formed a two-dimensional lattice. Further monomer additions to their sides generated a dense layer of oriented filaments that fully covered the lipid membrane. After this point the surface became unstable and the bilayer detached from the surface. Filame

SUBMITTER: Marquez I 

PROVIDER: S-EPMC6566678 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature