Neutrophil elastase correlates with increased sphingolipid content in cystic fibrosis sputum.

ABSTRACT:

Introduction

Sphingolipids are associated with the regulation of pulmonary inflammation. Although sphingolipids have been investigated in the context of cystic fibrosis (CF), the focus has been on loss of CF transmembrane conductance regulator (CFTR) function in mice, and in CF human lung epithelial cell lines. The sphingolipid content of CF sputum and the potential link between ceramide and airway inflammation in CF remain relatively unexplored.

Methods

Fifteen patients with CF provided two spontaneously expectorated sputum samples, one collected during a hospitalization for an acute pulmonary exacerbation and one from an outpatient visit at a time of clinical stability. Sputum was processed, and the supernatant assessed for active neutrophil elastase (NE) using a chromogen

SUBMITTER: Karandashova S 

PROVIDER: S-EPMC6566867 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature