Kinase Inhibitors with Antiepileptic Properties Identified with a Novel in Vitro Screening Platform.

ABSTRACT: Kinase signaling plays an important role in acquired epilepsy, but only a small percentage of the total kinome has been investigated in this context. A major roadblock that prevents the systematic investigation of the contributions of kinase signaling networks is the slow speed of experiments designed to test the chronic effects of target inhibition in epilepsy models. We developed a novel in vitro screening platform based on microwire recordings from an organotypic hippocampal culture model of acquired epilepsy. This platform enables the direct, parallel determination of the effects of compounds on spontaneous epileptiform activity. The platform also enables repeated recordings from the same culture over two-week long experiments. We screened 45 kinase inhibitors and quantified their effe

SUBMITTER: Liu J 

PROVIDER: S-EPMC6566965 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature