Safety and efficacy of ex vivo expanded CD34⁺ stem cells in murine and primate models.

ABSTRACT:

Background

Hematopoietic stem cell (HSC) transplantation has been widely applied to the treatment of malignant blood diseases. However, limited number of functional HSCs hinders successful transplantation. The purpose of our current study is to develop a new and cost-efficient medium formulation that could greatly enhance the expansion of HSCs while retaining their long-term repopulation and hematopoietic properties for effective clinical transplantation.

Methods

Enriched human CD34⁺ cells and mobilized nonhuman primate peripheral blood CD34⁺ cells were expanded with a new, cost-efficient expansion medium formulation, named hematopoietic expansion medium (HEM), consisting of various cytokines and nutritional supplements. The long-term repopulation poten

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6567473 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature