The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis.

ABSTRACT:

Background

Microbial mutagenesis is an important avenue to acquire microbial strains with desirable traits for industry application. However, mutagens either chemical or physical used often leads narrow library pool due to high lethal rate. The T4 DNA ligase is one of the most widely utilized enzymes in modern molecular biology. Its contribution to repair chromosomal DNA damages, therefore cell survival during mutagenesis will be discussed.

Results

Expression of T4 DNA ligase in vivo could substantially increase cell survival to ionizing radiation in multiple species. A T4 mediated survival-coupled mutagenesis approach was proposed. When polyhydroxybutyrate (PHB)-producing E. coli with T4 DNA ligase expressed in vivo was subjected to ionizing radiation, mutants with improved

SUBMITTER: Wang J 

PROVIDER: S-EPMC6567493 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature