Improving sensitivity of amyloid detection by Congo red stain by using polarizing microscope and avoiding pitfalls.

ABSTRACT: Systemic amyloidosis is a devastating group of disorders for which there is no current cure. The treatment goal is to reduce the burden of amyloidogenic protein precursors. The treatment is only effective if applied early in the disease process before significant and irreversible end organ damage has taken place. Congo red is still the standard stain used in most histopathology laboratories to identify amyloid material in tissues. The identification of Congophilic amyloid material is challenging because of multiple interfering factors. Here we describe improved sensitivity of identifying Congophilic materials in histologic sections using a metallurgical polarized microscope specifically constructed for polarized microscopy. The microscope is equipped with strain-free optics, matching polar

SUBMITTER: El-Meanawy A 

PROVIDER: S-EPMC6567537 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature