PhIP-Seq characterization of serum antibodies using oligonucleotide-encoded peptidomes.

ABSTRACT: The binding specificities of an individual's antibody repertoire contain a wealth of biological information. They harbor evidence of environmental exposures, allergies, ongoing or emerging autoimmune disease processes, and responses to immunomodulatory therapies, for example. Highly multiplexed methods to comprehensively interrogate antibody-binding specificities have therefore emerged in recent years as important molecular tools. Here, we provide a detailed protocol for performing 'phage immunoprecipitation sequencing' (PhIP-Seq), which is a powerful method for analyzing antibody-repertoire binding specificities with high throughput and at low cost. The methodology uses oligonucleotide library synthesis (OLS) to encode proteomic-scale peptide libraries for display on bacteriophage. These

SUBMITTER: Mohan D 

PROVIDER: S-EPMC6568263 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature