Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots.

ABSTRACT:

Background

The honeybee (Apis mellifera) has been used with great success in a variety of behavioral studies. The lack of genomic tools in this species has, however, hampered efforts to provide genome-based explanations for behavioral data. We have combined the power of DNA arrays and the availability of distinct behavioral stages in honeybees to explore the dynamics of gene expression during adult development in this insect. In addition, we used caffeine treatment, a procedure that accelerates learning abilities in honeybees, to examine changes in gene expression underlying drug-induced behavioral modifications.

Results

Spotted microarrays containing several thousand cDNAs were interrogated with RNAs extracted from newly emerged worker bees, experienced foragers and caffein

SUBMITTER: Kucharski R 

PROVIDER: S-EPMC65684 | biostudies-literature | 2002

REPOSITORIES: biostudies-literature