Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis is developed to be an attractive expression host to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. Chromosomal integration is a stable expression strategy while the expression level is not ideal compared with plasmid expression. Thus, to meet the requirement of protein overexpression, promoter, as one of the key elements, is important. It is necessary to obtain an ideal promoter for overproduction of foreign proteins from a single copy expression cassette.

Results

The activity of promoter P_ylb was further enhanced by optimizing the - 35, - 10 core region and upstream sequence (UP) by substituting both sequences with consensus sequences. The final engineered promoter exhibited almost 26

SUBMITTER: Zhou C 

PROVIDER: S-EPMC6570832 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature