MiR-520d-5p can reduce the mutations in hepatoma cancer cells and iPSCs-derivatives.

ABSTRACT:

Background

Human microRNAs (miRNAs) have diverse functions in biology, and play a role in nearly every biological process. Here we report that miR-520d-5p (520d-5p) causes undifferentiated cancer cells to adopt benign or normal status in vivo in immunodeficient mice via demethylation and P53 upregulation. Further we found that 520-5p causes normal cells to elongate cellular lifetime and mesenchymal stem cell-like status with CD105 positivity. We hypothesized that ectopic 520d-5p expression reduced mutations in undifferentiated type of hepatoma (HLF) cells through synergistic modulation of methylation-related enzymatic expression.

Methods

To examine whether there were any changes in mutation status in cells treated with 520d-5p, we performed next generation sequencing (NGS) i

SUBMITTER: Miura N 

PROVIDER: S-EPMC6570841 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature