MTORC1 and mTORC2 are differentially engaged in the development of laser-induced CNV.

ABSTRACT:

Background

The mechanistic target of rapamycin (mTOR) pathway is a potential target to inhibit pathologic processes in choroidal neovascularization. However, the exact role of mTOR signaling in the development of CNV remains obscure. In this study, we assessed the role of mTORC1 and mTORC2 as well as the effect of rapamycin (sirolimus) on choroidal neovascularization (CNV) in a laser-induced mouse model.

Methods

In experiment A, we observed the natural course of CNV development and the dynamics of mTOR-related proteins during the 12 days after the laser injury. The expression of mTOR-related proteins was evaluated using Western blot (WB). Cryosections of CNV-induced mice were immunostained for the visualization of the vascular and extravascular components of the CNV. Experim

SUBMITTER: Yang JY 

PROVIDER: S-EPMC6570852 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature