Viral integration drives multifocal HCC during the occult HBV infection.

ABSTRACT:

Background & aims

Although the prognosis of patients with occult hepatitis B virus (HBV) infection (OBI) is usually benign, a small portion may undergo cirrhosis and subsequently hepatocellular carcinoma (HCC). We studied the mechanism of life-long Integration of virus DNA into OBI host's genome, of which may induce hepatocyte transformation.

Methods

We applied HBV capture sequencing on single cells from an OBI patient who, developed multiple HCC tumors and underwent liver resection in May 2013 at Tongji Hospital in China. Despite with the undetectable virus DNA in serum, we determined the pattern of viral integration in tumor cells and adjacent non-tumor cells and obtained the details of the viral arrangement in host genome, and furthermore the HBV integrated region in canc

SUBMITTER: Chen XP 

PROVIDER: S-EPMC6570863 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature