Interactional similarities and differences in the protein complex of PCNA and DNA replication factor C between rice and Arabidopsis.

ABSTRACT:

Background

Proliferating cell nuclear antigen (PCNA), a conserved trimeric ring complex, is loaded onto replication fork through a hetero-pentameric AAA+ ATPase complex termed replication factor C (RFC) to maintain genome stability. Although architectures of PCNA-RFC complex in yeast have been revealed, the functions of PCNA and protein-protein interactions of PCNA-RFC complex in higher plants are not very clear. Here, essential regions mediating interactions between PCNA and RFC subunits in Arabidopsis and rice were investigated via yeast-two-hybrid method and bimolecular fluorescence complementation techniques.

Results

We observed that OsPCNA could interact with all OsRFC subunits, while protein-protein interactions only exist between Arabidopsis RFC2/3/4/5 and AtPCNA1/2.

SUBMITTER: Qian J 

PROVIDER: S-EPMC6570896 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature