Prostaglandin F_2α (PGF_2α) production possibility and its receptors expression in the early- and late-cleaved preimplantation bovine embryos.

ABSTRACT:

Background

Prostaglandin F_2α (PGF_2α) is an important component for the physiology of female reproductive processes. In the literature, the data pertaining to the synthesis and action of PGF_2α in early embryonic bovine development are limited. In our study, we used the bovine in vitro culture model based on the time of first cleavage to determine the mRNA expression and immunolocalization of PGF_2α synthase and its receptor in bovine embryos from the 2-cell stage to the hatched blastocyst stage. We also evaluated PGF_2α production at 2, 5 and 7 days of in vitro culture.

Results

We found a significantly higher proportion of blastocysts obtained from the early-cleaved embryos than from the late-cleaved embryos (37.7% vs. 26.1

SUBMITTER: Grycmacher K 

PROVIDER: S-EPMC6570898 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature