Molecular evidence of Tula virus in Microtus obscurus in the region of Yili, Xinjiang, China.

ABSTRACT:

Background

Hantaviruses are important zoonotic pathogens, and they pose a profound risk to public health. So far, there has been no evidence showing that Tula virus (TULV), one species of hantavirus, is endemic in China. In this study, we captured rodents and found that the Tula virus had infected voles in Yili region, Xinjiang, China.

Methods

Rodents were captured by flooding their burrows in mountain pasture areas in Narati, Xinyuan County, Xinjiang, China. Hantavirus L gene fragments were amplified by nest RT-PCR using genus-specific primers. Positive samples were further identified by sequencing of RT-PCR products of S gene fragment for species identification. To identify the species of captured small mammals, the rodents' cytochrome b (Cytb) was amplified by PCR and seq

SUBMITTER: Guo G 

PROVIDER: S-EPMC6570900 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature