SP2: Rapid and Automatable Contaminant Removal from Peptide Samples for Proteomic Analyses.

ABSTRACT: Peptide cleanup is essential for the removal of contaminating substances that may be introduced during sample preparation steps in bottom-up proteomic workflows. Recent studies have described benefits of carboxylate-modified paramagnetic particles over traditional reversed-phase methods for detergent and polymer removal, but challenges with reproducibility have limited the widespread implementation of this approach among laboratories. To overcome these challenges, the current study systematically evaluated key experimental parameters regarding the use of carboxylate-modified paramagnetic particles and determined those that are critical for maximum performance and peptide recovery and those for which the protocol is tolerant to deviation. These results supported the development of a detaile

SUBMITTER: Waas M 

PROVIDER: S-EPMC6571012 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature