Modulation of phospho-proteins by interferon-alpha and valproic acid in acute myeloid leukemia.

ABSTRACT:

Purpose

Valproic acid (VPA) is suggested to be therapeutically beneficial in combination with interferon-alpha (IFNα) in various cancers. Therefore, we examined IFNα and VPA alone and in combinations in selected AML models, examining immune regulators and intracellular signaling mechanisms involved in phospho-proteomics.

Methods

The anti-leukemic effects of IFNα and VPA were examined in vitro and in vivo. We mapped the in vitro phosphoprotein modulation by IFNα-2b and human IFNα-Le in MOLM-13 cells by IMAC/2D DIGE/MS analysis and phospho-flow cytometry, and in primary healthy and AML patient-derived PBMCs by CyTOF. In vivo, IFNα-Le and VPA efficacy were investigated in the immunodeficient NOD/Scid IL2γ-/- MOLM-13^Luc+ mouse model and the syngeneic immunocompetent B

SUBMITTER: Forthun RB 

PROVIDER: S-EPMC6571093 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature