The effects of PTPN2 loss on cell signalling and clinical outcome in relation to breast cancer subtype.

ABSTRACT:

Purpose

The protein tyrosine phosphatase PTPN2 dephosphorylates several tyrosine kinases in cancer-related signalling pathways and is thought to be a tumour suppressor. As PTPN2 is not frequently studied in breast cancer, we aimed to explore the role of PTPN2 and the effects of its loss in breast cancer.

Methods

Protein expression and gene copy number of PTPN2 were analysed in a cohort of pre-menopausal breast cancer patients with immunohistochemistry and droplet digital PCR, respectively. PTPN2 was knocked down in three cell lines, representing different breast cancer subtypes, with siRNA transfection. Several proteins related to PTPN2 were analysed with Western blot.

Results

Low PTPN2 protein expression was found in 50.2% of the tumours (110/219), gene copy loss in

SUBMITTER: Veenstra C 

PROVIDER: S-EPMC6571101 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature