Effects of Central Loop Length and Metal Ions on the Thermal Stability of G-Quadruplexes.

ABSTRACT: The central loop of G-quadruplex molecular beacons is a key element to sense target DNA or RNA sequences. In this study, circular dichroism spectroscopy (CD), thermal difference spectrum (TDS), non-denatured non-denaturing gel electrophoresis, and thermal stability analysis were used to investigate the effect of the central loop length on G-quadruplex features. Two series of G-quadruplexes, AG₃TTAG₃-(TTA)n-G₃TTAG₃T (n = 1-8) (named TTA series) and AG₃TTTG₃-(TTA)n-G₃TTTG₃T (n = 1-8) (named TTT series) were examined in K⁺ and Na⁺ solutions, respectively. CD and TDS spectral data indicated that TTA series adopted an antiparallel G-quadruplex structure in Na⁺ s

SUBMITTER: Hao F 

PROVIDER: S-EPMC6571788 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature