Effects of Long-Term Storage at -80 °C on the Human Plasma Metabolome.

ABSTRACT: High-quality biological samples are required for the favorable outcome of research studies, and valid data sets are crucial for successful biomarker identification. Prolonged storage of biospecimens may have an artificial effect on compound levels. In order to investigate the potential effects of long-term storage on the metabolome, human ethylenediaminetetraacetic acid (EDTA) plasma samples stored for up to 16 years were analyzed by gas and liquid chromatography-tandem mass spectrometry-based metabolomics. Only 2% of 231 tested plasma metabolites were altered in the first seven years of storage. However, upon longer storage periods of up to 16 years and more time differences of few years significantly affected up to 26% of the investigated metabolites when analyzed within subject age grou

SUBMITTER: Wagner-Golbs A 

PROVIDER: S-EPMC6572224 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature