Embossed Membranes with Vascular Patterns Guide Vascularization in a 3D Tissue Model.

ABSTRACT: The vascularization of three-dimensional (3D) tissue constructs is necessary for transporting nutrients and oxygen to the component cells. In this study, a vacuum forming method was applied to emboss a vascular pattern on an electrospun membrane so that guided vascular structures could develop within the construct. Two- or six-layer constructs of electrospun membranes seeded with endothelial cells and pericytes were stacked and subcutaneously implanted into mice. Blood vessel formation in the implanted constructs with six alternating layers of flat membranes and membranes embossed with a blood vessel pattern was observed after two weeks of implantation. The formation of blood vessels was observed along the embossed blood vessel pattern in the structure of the embossed membrane laminated at

SUBMITTER: Hong S 

PROVIDER: S-EPMC6572394 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature