Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells.

ABSTRACT: Dyes with environment-sensitive fluorescence have proven useful to study the spatiotemporal dynamics of protein activity in living cells. When attached to proteins, their fluorescence can reflect protein conformational changes, post-translational modifications, or protein interactions. However, the utility of such dye-protein conjugates has been limited because it is difficult to load them into cells. They usually must be introduced using techniques that perturb cell physiology, limit throughput, or generate fluorescent vesicles (e.g., electroporation, microinjection, or membrane transduction peptides). Here we circumvent these problems by modifying a proven, environment-sensitive biosensor fluorophore so that it can pass through cell membranes without staining intracellular compartments a

SUBMITTER: MacNevin CJ 

PROVIDER: S-EPMC6572722 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature