Ontology highlight
ABSTRACT:
SUBMITTER: Ro SY
PROVIDER: S-EPMC6572826 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Nature communications 20190617 1
Aerobic methane oxidation is catalyzed by particulate methane monooxygenase (pMMO), a copper-dependent, membrane metalloenzyme composed of subunits PmoA, PmoB, and PmoC. Characterization of the copper active site has been limited by challenges in spectroscopic analysis stemming from the presence of multiple copper binding sites, effects of detergent solubilization on activity and crystal structures, and the lack of a heterologous expression system. Here we utilize nanodiscs coupled with native t ...[more]