NAD tagSeq reveals that NAD⁺-capped RNAs are mostly produced from a large number of protein-coding genes in Arabidopsis.

ABSTRACT: The 5' end of a eukaryotic mRNA transcript generally has a 7-methylguanosine (m⁷G) cap that protects mRNA from degradation and mediates almost all other aspects of gene expression. Some RNAs in Escherichia coli, yeast, and mammals were recently found to contain an NAD⁺ cap. Here, we report the development of the method NAD tagSeq for transcriptome-wide identification and quantification of NAD⁺-capped RNAs (NAD-RNAs). The method uses an enzymatic reaction and then a click chemistry reaction to label NAD-RNAs with a synthetic RNA tag. The tagged RNA molecules can be enriched and directly sequenced using the Oxford Nanopore sequencing technology. NAD tagSeq can allow more accurate identification and quantification of NAD-RNAs, as well as reveal the sequ

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6575590 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature